Autofluorescence

Autofluorescence

Some materials fluoresce under UV or blue excitation, releasing light at longer wavelengths. This autofluorescence creates a non-coherent, unstructured signal, which can contaminate imaging data—particularly in fluorescence microscopy, biosensing, and quantum applications.

Common in:

  • Acrylate-based epoxies
  • Lower-grade polymers

 

Minimized in:

  • Optical-grade silicones
  • UV-stabilized polymers
  • Specialty low-fluorescence epoxies

 

Test Method:
UV excitation with a photodetector or a hyperspectral camera to analyze emitted background

 

Mitigation:

  • Choose resins designed for low autofluorescence
  • Minimize post-curing thermal oxidation (can increase fluorescence over time)
  • Spec Expectation: Often qualitative or comparative unless part of fluorescence imaging application stack.